NY96015 - Detection and Characterisation of Orchid Fleck Virus

The main objective of this project was o develop a nucleic acid based diagnostic system for orchid fleck virus (OFV). OFV is one of three commonly found viruses infecting orchids. Diagnosis of virus diseases are commonly conducted by immunological tests but this method is not available for use with OFV because OFV has a very unstable virion. A RT-PCR test was developed that reliably detects OFV specifically in fresh or frozen leaf tissue. The RT-PCR test can now be utilized by orchid growers and nurseries through the appropriate testing agencies.